Histochemical analysis of lymphatic endothelial cells in lymphostasis
Identifieur interne : 009F08 ( Main/Exploration ); précédent : 009F07; suivant : 009F09Histochemical analysis of lymphatic endothelial cells in lymphostasis
Auteurs : Rui-Cheng Ji [Japon] ; Seiji Kato [Japon]Source :
- Microscopy Research and Technique [ 1059-910X ] ; 2001-10-15.
Abstract
The ultrastructure of endothelial cells of intestinal lymphatics and the thoracic duct (TD) and the relation to lymphostasis were examined in rats and monkeys. Localization of 5′‐nucleotidase (5′‐Nase) and endothelial nitric oxide synthase (eNOS) was studied. In normal lymphatic endothelial cells, 5′‐Nase reaction product was evenly deposited on the cell surface in vivo and on cultured TD endothelial cells (TDECs), whereas eNOS was evenly distributed throughout the nucleus and cytoplasm. TDECs had a long filamentous process extending towards the subendothelial extracellular matrix but became flat and regular within 30–40 minutes after gastric perfusion with olive oil. According to their electron‐density, two types of cells were found in the TD endothelial layer. The cells with low electron‐density exhibited stronger 5′‐Nase activity. Valves were bicuspid formations and the valvular endothelial surface of the convex side showed weaker 5′‐Nase activity than the concave side. During TD blockage‐induced lymphostasis in rats, the 5′‐Nase product was almost not discernible in the TDECs within 2 weeks. Larger vesicles were found in the endothelial cytoplasm of the ligated TD. Their number decreased after 6–12 weeks. The small intestinal lymphatics in the mucosa and submucosa were dilated, with numerous open intercellular junctions. The endothelial lining appeared to have reduced activities for 5′‐Nase and eNOS in 9 of 11 experimental animals. The results indicated that the inability of the open intercellular junctions, normally working as one‐way endothelial flap valves, may be a key morphological feature after TD blockage. Reduced eNOS and 5′‐Nase may functionally influence contractile activity and transport capability of the lymphatic vessels in the lymphostasis. Microsc. Res. Tech. 55:70–80, 2001. © 2001 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jemt.1158
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 004702
- to stream Istex, to step Curation: 004702
- to stream Istex, to step Checkpoint: 002C12
- to stream Main, to step Merge: 00A362
- to stream Main, to step Curation: 009F08
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Histochemical analysis of lymphatic endothelial cells in lymphostasis</title><author><name sortKey="Ji, Rui Heng" sort="Ji, Rui Heng" uniqKey="Ji R" first="Rui-Cheng" last="Ji">Rui-Cheng Ji</name></author><author><name sortKey="Kato, Seiji" sort="Kato, Seiji" uniqKey="Kato S" first="Seiji" last="Kato">Seiji Kato</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:9639DEE22BEBA821FA89C096FCFFC9DC44E3EF06</idno><date when="2001" year="2001">2001</date><idno type="doi">10.1002/jemt.1158</idno><idno type="url">https://api.istex.fr/document/9639DEE22BEBA821FA89C096FCFFC9DC44E3EF06/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">004702</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">004702</idno><idno type="wicri:Area/Istex/Curation">004702</idno><idno type="wicri:Area/Istex/Checkpoint">002C12</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002C12</idno><idno type="wicri:doubleKey">1059-910X:2001:Ji R:histochemical:analysis:of</idno><idno type="wicri:Area/Main/Merge">00A362</idno><idno type="wicri:Area/Main/Curation">009F08</idno><idno type="wicri:Area/Main/Exploration">009F08</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Histochemical analysis of lymphatic endothelial cells in lymphostasis</title><author><name sortKey="Ji, Rui Heng" sort="Ji, Rui Heng" uniqKey="Ji R" first="Rui-Cheng" last="Ji">Rui-Cheng Ji</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Anatomy, Oita Medical University, Oita 879‐5593</wicri:regionArea><wicri:noRegion>Oita 879‐5593</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Anatomy, Oita Medical University, Oita 879‐5593</wicri:regionArea><wicri:noRegion>Oita 879‐5593</wicri:noRegion></affiliation></author><author><name sortKey="Kato, Seiji" sort="Kato, Seiji" uniqKey="Kato S" first="Seiji" last="Kato">Seiji Kato</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Anatomy, Oita Medical University, Oita 879‐5593</wicri:regionArea><wicri:noRegion>Oita 879‐5593</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Microscopy Research and Technique</title><title level="j" type="sub">Biology of Lymphangiogenesis</title><title level="j" type="alt">MICROSCOPY RESEARCH AND TECHNIQUE</title><idno type="ISSN">1059-910X</idno><idno type="eISSN">1097-0029</idno><imprint><biblScope unit="vol">55</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="70">70</biblScope><biblScope unit="page" to="80">80</biblScope><biblScope unit="page-count">11</biblScope><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="2001-10-15">2001-10-15</date></imprint><idno type="ISSN">1059-910X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1059-910X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The ultrastructure of endothelial cells of intestinal lymphatics and the thoracic duct (TD) and the relation to lymphostasis were examined in rats and monkeys. Localization of 5′‐nucleotidase (5′‐Nase) and endothelial nitric oxide synthase (eNOS) was studied. In normal lymphatic endothelial cells, 5′‐Nase reaction product was evenly deposited on the cell surface in vivo and on cultured TD endothelial cells (TDECs), whereas eNOS was evenly distributed throughout the nucleus and cytoplasm. TDECs had a long filamentous process extending towards the subendothelial extracellular matrix but became flat and regular within 30–40 minutes after gastric perfusion with olive oil. According to their electron‐density, two types of cells were found in the TD endothelial layer. The cells with low electron‐density exhibited stronger 5′‐Nase activity. Valves were bicuspid formations and the valvular endothelial surface of the convex side showed weaker 5′‐Nase activity than the concave side. During TD blockage‐induced lymphostasis in rats, the 5′‐Nase product was almost not discernible in the TDECs within 2 weeks. Larger vesicles were found in the endothelial cytoplasm of the ligated TD. Their number decreased after 6–12 weeks. The small intestinal lymphatics in the mucosa and submucosa were dilated, with numerous open intercellular junctions. The endothelial lining appeared to have reduced activities for 5′‐Nase and eNOS in 9 of 11 experimental animals. The results indicated that the inability of the open intercellular junctions, normally working as one‐way endothelial flap valves, may be a key morphological feature after TD blockage. Reduced eNOS and 5′‐Nase may functionally influence contractile activity and transport capability of the lymphatic vessels in the lymphostasis. Microsc. Res. Tech. 55:70–80, 2001. © 2001 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Ji, Rui Heng" sort="Ji, Rui Heng" uniqKey="Ji R" first="Rui-Cheng" last="Ji">Rui-Cheng Ji</name></noRegion><name sortKey="Ji, Rui Heng" sort="Ji, Rui Heng" uniqKey="Ji R" first="Rui-Cheng" last="Ji">Rui-Cheng Ji</name><name sortKey="Kato, Seiji" sort="Kato, Seiji" uniqKey="Kato S" first="Seiji" last="Kato">Seiji Kato</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 009F08 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 009F08 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:9639DEE22BEBA821FA89C096FCFFC9DC44E3EF06
|texte= Histochemical analysis of lymphatic endothelial cells in lymphostasis
}}
| This area was generated with Dilib version V0.6.31. |  |